Induction medium for regeneration of aralia elata leaf plants

• Main Authors: LIANG GUIRONG, LAN ZHIHUA, WANG XINHUA, SONG XIAOHONG, ZHAO HENGTIAN, SHEN YUNXIA
• Format: Patent
• Language: Chinese; English
• Published: 28.07.2010
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRAPPING; VINEGAR; WINE

The present invention belongs to the technical field of plant tissue culture during the agricultural production and is an Aralia eleta leaf culture and a plant of Aralia eleta regeneration induction culture medium. The present invention comprises culture medium nutrient element and culture medium hormone, wherein culture medium inorganic salt comprises NH4NO3, NH4H2PO4, KNO3, CaCl2.2H2O, MgSO4.7H2O, Ca(NO3)2.4H2O, EDTA-Fe, KI, MnSO4.H2O, H3BO3, ZnSO4.7H2O, NaMoO4.2H2O, CuSO4.5H2O and CoCl2.6H2O; the hormone comprises BA, IAA, NAA and ZT. The concentration of the inorganic salt contained by the present invention is quite low, which can save the cost nearly two times; the suitable hormone matching and species can effectively promote callus differentiation and the regeneration and the proliferation of leaf bud. The regeneration plant induction rate of the culture medium laminae of the present invention is more than 75 percent.