Recombination human ciliary neurotrophy factor, mutant and application thereof

• Main Authors: JIANG LIN, MA YARU, YING LIANFANG, YANG JUN, XIN FANG, LEI QING, LIANG LINGYU, XIE QIN
• Format: Patent
• Language: Chinese; English
• Published: 05.09.2012
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

The present invention relates to a recombinant human ciliary nerve nutrition factor, a mutant of the nutrition factor, and the preparation and the application thereof. The present invention provides the recombinant human ciliary nerve nutrition factor that the 17th thioserine of a natural CNTF gene is changed into alanine, the 63rd glutamic acid of the protogene is changed into arginine, at the same time, the partial codons in the CNTF protogene are changed into the codons which is favored by prokaryotes, but the code of the gene is not changed. On the basis, the recombinant CNTF is modified,to form different mutants. The mutants of the present invention has better effect on the aspect of curing the ordinary obesity or diabetes type obesity.