Process for producing erysipelothrix rhusiopathiae surface protective antigen mutant in escherichia coli

• Main Author: USHIJIMA TOSHIHIRO,SAKAGUCHI MASASHI,TOKUNAGA EIJI
• Format: Patent
• Language: English
• Published: 05.09.2007
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

A variant of Erysipelothrix rhusiopathiae surface protective antigen SpaA protein or of a shortened form of SpaA ( SpaA) in which a portion of SpaA protein is deleted for protection from Erysipelothrix rhusiopathiae infection and a process for preparing the same are provided. Introduction of amino acid substitution at a specific site in the amino acid sequence of SpaA or SpaA protein provides a variant of SpaA or SpaA protein which is immunogenic and is expressed in E. coli as inclusion bodies. The variant of SpaA or SpaA protein of the present invention may easily be recovered and purified since it is expressed in E. coli as inclusion bodies.It is intended to provide a mutant of a surface protective antigen SpaA protein of Erysipelothrix rhusiopathiae or a shortened SpaA protein (DeltaSpaA) obtained by removing a part therefrom and a process for producing the same. By introducing an amino acid substituent into a definite position of the amino acid sequence of the SpaA protein or the DeltaSpaA protein, a mutant of the SpaA protein or the DeltaSpaA protein having immunogenicity and being expressed as an inclusion body in Escherichia coli can be obtained. Because of being expressed as an insoluble inclusion body in Escherichia coli, the above-described mutant of the SpaA protein or the DeltaSpaA protein can be easily collected and purified.