Gene chip for detection and typing of bird flu virus

• Main Author: XUEQING,LIN HAN
• Format: Patent
• Language: English
• Published: 22.08.2007
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

The present invention relates to gene chip for the detection and subtyping of bird flu virus. The present invention establishes gene chip with high specificity, high sensitivity and great practicability for detection and subtyping of bird flu virus through designing and screening 50 pairs of specific primers, 2 universal primers, 52 specific probes and 3 quality control probes for the multiple RT-PCR subtyping detection of bird flu virus genes based on the gene sequences of different bird flu virus subtypes Genebank reports; and performing the effectiveness test, specificity test, sensitivity test and condition optimization of gene chip detection. The present invention establishes also the detection and subtyping process with the chip and gives the initial application.