Multitargeting bispecific antigen-binding molecules of increased selectivity

• Main Authors: Claudia BLUEMEL, Julie BAILIS, MUENZ, Markus, Peter KUFER, Petra LUTTERBUESE, Sascha WEIDLER, Igor D'ANGELO, Oliver THOMAS, Virginie NAEGELE, Doris RAU, Johannes BROZY, Ines ULLRICH, Christian WEBHOFER, Benno RATTEL, Joachim WAHL, Adam ZALEWSKI, Stephanie EVERTS, Matthias KLINGER, Thomas BOEHM, Tobias RAUM, Elizabeth PHAM
• Format: Patent
• Language: English; Spanish
• Published: 19.01.2024
• Subjects: CHEMISTRY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; ORGANIC CHEMISTRY; PEPTIDES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES

La presente invención proporciona moléculas de unión a antígeno biespecíficas con múltiples dianas caracterizadas por comprender una primera y una segunda entidad biespecífica, comprendiendo cada una de las cuales un dominio que se une a la diana, y un segundo dominio que se une a un epítopo extracelular de la cadena CD3? humana y de Macaca, en donde ambas entidades biespecíficas están unidas entre sí por un espaciador que separa la primera y la segunda entidad biespecífica. Además, la invención proporciona un polinucleótido que codifica la molécula de unión a antígeno biespecífica con múltiples dianas, un vector que comprende este polinucleótido, células hospedadores que expresan la construcción, así como una composición farmacéutica que comprende el mismo. The present invention provides multitargeting bispecific antigen-binding molecules characterized by comprising a first and a second bispecific entity each comprising a domain binding to target, a second domain binding to an extracellular epitope of the human and the Macaca CD3e chain, wherein both bispecific entities are linked to each other by a spacer which spaces apart the first and the second bispecific entity. Moreover, the invention provides a polynucleotide, encoding the multitargeting bispecific antigen-binding molecule, a vector comprising this polynucleotide, host cells, expressing the construct and a pharmaceutical composition comprising the same. The present invention provides multitargeting bispecific antigen-binding molecules characterized by comprising a first and a second bispecific entity each comprising a domain binding to target, a second domain binding to an extracellular epitope of the human and the Macaca CD3ε chain, wherein both bispecific entities are linked to each other by a spacer which spaces apart the first and the second bispecific entity. Moreover, the invention provides a polynucleotide, encoding the multitargeting bispecific antigen-binding molecule, a vector comprising this polynucleotide, host cells, expressing the construct and a pharmaceutical composition comprising the same.