PROCEDIMIENTO PARA OBTENER PROTEINA TBPB EN LA SUPERFICIE DE UNA BACTERIA GRAM NEGATIVA QUE COMPRENDE CONSTRUIR UN PLASMIDO PET CON EL GEN TBPB INCORPORADO; PROTEINA TBPB; ANTIGENO TBPB DE LA CEPA CHILENA NEISSERIA MENINGITIDIS B:4:NT; GEN TBPB DE LA

• Main Author: ENRIQUE A. ROMAN ESPINOZA, ANDRES REGHEZZA INZUNZA, RAMON DEL RIO BAEZ
• Format: Patent
• Language: Spanish
• Published: 02.05.2008
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS; PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRAPPING; VINEGAR; WINE

A procedure for obtaining the expression of a membrane antigen of a pathogen against which an live oral vaccine development is desirable on the surface of a negative Gram bacteria to which virulence is attenuated, or another bacteria or other Gram negative or positive bacteria with probiotic features which are compatible with the proposed expression system and that can be used as a live oral vaccine, wherein a plasmid is constructed and obtained based on the structure of pET family plasmids, with tbpB gene incorporated under the control of T7 promoter or another equivalent one, with the addition of a metabolic marker in the plasmid vector, previously cloned with its own promoter, inactivating, at the same time, the antibiotic resistance. In addition, recombinant microorganism such as an attenuated vaccine strain against group B meningitis with immunizing and protective properties against infection by Neisseria meningitidis.