IN-VITRO-VERFAHREN ZUR DIAGNOSE VON MALIGNEN TUMOREN

An in vitro testing method for the diagnosis of malignant tumours preferably human tumours, by determining the mobility variation of cellular blood components contacted with a migration inhibition factor (MIF), the MIF being secreted from lymphocytes being sensitized against tumour tissue, comprises...

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Bibliographic Details
Main Author DOUWES, FRIEDRICH
Format Patent
LanguageGerman
Published 14.12.1984
Subjects
DIAGNOSIS
HUMAN NECESSITIES
HYGIENE
IDENTIFICATION
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
MEDICAL OR VETERINARY SCIENCE
PHYSICS
SURGERY
TESTING
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Summary:An in vitro testing method for the diagnosis of malignant tumours preferably human tumours, by determining the mobility variation of cellular blood components contacted with a migration inhibition factor (MIF), the MIF being secreted from lymphocytes being sensitized against tumour tissue, comprises subjecting, as said cellular blood components, the lymphocytes contained in a leucocyte suspension to be examined to a migration inhibition test. The lymphocytes are contacted with an antigen capable of stimulating them to produce MIF. The leucocyte migration is inhibited and fixed after a predetermined period of time. The leucocytes migrate in a capillary intermediate layer having a thickness of from 1 to 20 microns and being at least partially filled with liquid. Said intermediate layer is formed between a glass surface and an agar layer. The invention further refers to the construction and preparation of a testing plate or dish comprising these layers.
Bibliography:Application Number: CH19780012329