RNASE H2 MUTANTS THAT REDUCE PRIMER DIMERS AND OFF-TARGET AMPLIFICATION IN RHPCR-BASED AMPLICON SEQUENCING WITH HIGH-FIDELITY DNA POLYMERASES

• Main Authors: BELTZ, KRISTIN, ROSE, SCOTT, PERSCHBACHER, KATHERINE, BEHLKE, MARK AARON, FROEHLIG, JOHN, DOBOSY, JOSEPH
• Format: Patent
• Language: English; French
• Published: 30.06.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The present invention pertains to hybrid RNase H2 proteins that include fragments of amino acid sequences from Pyrococcus abyssi ( P.a.), Thermococcus kodakarensis (T.kod), and Pyrococcus furiosus organisms, as well as methods of using the same to improve mismatch discrimination and activity in a high-fidelity DNA polymerase buffer. La présente invention concerne des protéines RNase H2 hybrides comprenant des fragments de séquences d'acides aminés provenant d'organismes Pyrococcus abyssi ( P.a.), Thermococcus kodakarensis (T.kod), et Pyrococcus furiosus, ainsi que des procédés d'utilisation de ces derniers pour améliorer la discrimination des mésappariements et l'activité dans un tampon d'ADN polymérase haute fidélité.