DNA-CONTAINING POLYNUCLEOTIDES AND GUIDES FOR CRISPR TYPE V SYSTEMS, AND METHODS OF MAKING AND USING THE SAME

The present disclosure provides guides for use in Type V CRISPR systems wherein such guides contain ribonucleotide bases and at least one deoxyribonucleotide base. CRISPR Casl2 guides that contain at least one deoxyribonucleotide base, as well as nucleoprotein complexes of Type V CRISPR-Casl2 protei...

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Bibliographic Details
Main Author DONOHOUE, PAUL DANIEL
Format Patent
LanguageEnglish
French
Published 28.04.2022
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:The present disclosure provides guides for use in Type V CRISPR systems wherein such guides contain ribonucleotide bases and at least one deoxyribonucleotide base. CRISPR Casl2 guides that contain at least one deoxyribonucleotide base, as well as nucleoprotein complexes of Type V CRISPR-Casl2 proteins and such guides, are also described. Also disclosed are methods for making and using the deoxyribonucleotide-containing polynucleotides and guides, and for making and using the nucleoprotein complexes. Also disclosed are methods for engineering cells using Cast 2 chRDNA guide/nucl eoprotein complexes to produce CAR-expressing cells; and the use of such CAR -expressing cells in adaptive cell therapy. La présente divulgation concerne des guides destinés à être utilisés dans des systèmes CRISPR de type V, ces guides contenant des bases ribonucléotidiques et au moins une base désoxyribonucléotidique. La divulgation concerne également des guides de Casl2 CRISPR qui contiennent au moins une base désoxyribonucléotidique, ainsi que des complexes de nucléoprotéines de protéines CRISPR-Casl2 de type V et de tels guides. L'invention divulgue également des méthodes de fabrication et d'utilisation des polynucléotides et des guides contenant des désoxyribonucléotides, et de fabrication et d'utilisation des complexes de nucléoprotéines. L'invention divulgue en outre des méthodes de modification de cellules à l'aide de guides d'ADNRch Cast 2/de complexes de nucléoprotéines pour produire des cellules exprimant CAR ; et l'utilisation de ces cellules exprimant CAR dans une thérapie cellulaire adaptative.
Bibliography:Application Number: CA20213198905