BIOSYNTHESIS OF CANNABINOIDS AND CANNABINOID PRECURSORS

• Main Authors: RODRIGUEZ, GABRIEL, SPENCER, MICHELLE, CARLIN, DYLAN ALEXANDER, FLORES, NICHOLAS, FORREST, KATRINA, CARVALHO, BRIAN, SCHMIDT, NATHAN W
• Format: Patent
• Language: English; French
• Published: 21.04.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMBINATORIAL CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICOLIBRARIES; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

Aspects of the disclosure relate to biosynthesis of cannabinoids and cannabinoid precursors in recombinant cells and in vitro. Specifically, the disclosure is directed to a prenyltransferase variant, a chimeric prenyltransferase comprising one or more portions of at least two different prenyltransferase proteins, and a fusion polypeptide comprising a CBG-type producing prenyltransferase and a farnesyl pyrophosphate synthase. Des aspects de l'invention concernent la biosynthèse de cannabinoïdes et de précurseurs de cannabinoïdes dans des cellules de recombinaison et in vitro. En particulier, l'invention concerne une variante de prényltransférase, une prényltransférase chimère comprenant une ou plusieurs parties d'au moins deux protéines de prényltransférase différentes, et un polypeptide de fusion comprenant une prényltransférase de type CBG et une farnésyl-pyrophosphate synthase.