METHODS OF MAKING CHIMERIC ANTIGEN RECEPTOR-EXPRESSING CELLS

• Main Authors: GUIMARAES, CARLA PATRICIA PINTO, TREANOR, LOUISE, CARBONNEAU, SETH, KODRASI, OLJA, LIM, HYUNGWOOK, ZHU, XU, PRATICO, ELIZABETH DOROTHY, HACK, ANNIESHA, ENGELS, BORIS, SOHONI, AKASH, GREENE, MICHAEL R, SHARMA, SUJATA, PRICE, ANDREW, BROGDON, JENNIFER, HILD, MARC HORST PETER, DRANOFF, GLENN, STEIN, ANDREW MARC, BONDAZA, ATTILIO
• Format: Patent
• Language: English; French
• Published: 02.09.2021
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

This disclosure provides methods of making immune effector cells (for example, T cells, NK cells) that comprise (i) a nucleic acid molecule that encodes a controllable chimeric antigen receptor (CCAR) or (ii) a nucleic acid molecule that encodes a CAR and a regulatory molecule, and compositions generated by such methods. La présente invention concerne des procédés de production de cellules effectrices immunes (par exemple, des lymphocytes T, des cellules NK) qui comprennent (i) une molécule d'acide nucléique codant pour un récepteur antigénique chimérique pouvant être commandé (CCAR) ou (ii) une molécule d'acide nucléique codant pour un CAR et une molécule régulatrice, ainsi que des compositions générées selon de tels procédés.