STRAIN AND METHOD FOR PRODUCING ROSMARINIC ACID

• Main Authors: YAN, YI, CAI, YUJIE, DING, YANRUI, BAI, YAJUN, ZHENG, XIAOHUI
• Format: Patent
• Language: English; French
• Published: 30.12.2020
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

Provided are a strain and method for producing rosmarinic acid. Recombinant cells expressing 4-coumarate-CoA ligase, rosmarinic acid synthase, polyphosphate kinase 2-I, and polyphosphate kinase 2-II or combinations of the recombinant cells are constructed, and the recombinant cells or the combinations of the recombinant cells are used for catalyzing synthesis of rosmarinic acid from Danshensu and caffeic acid. La présente invention concerne une souche et un procédé de production d'acide rosmarinique. L'invention concerne des cellules recombinées exprimant la 4-coumarate-CoA ligase, la synthase d'acide rosmarinique, la polyphosphate kinase 2-I, et la polyphosphate kinase 2-II ou des combinaisons des cellules de recombinaison sont construites, et les cellules de recombinaison ou les combinaisons des cellules de recombinaison sont utilisées pour catalyser la synthèse de l'acide rosmarinique à partir de Danshensu et d'acide caféique.