GENERATION OF HUMAN PLURIPOTENT STEM CELL DERIVED ARTIFICIAL TISSUE STRUCTURES WITHOUT THREE DIMENSIONAL MATRICES

The present invention provides a differentiation medium for differentiation and expansion of a multicellular aggregation in suspension derived from human pluripotent stem cells that has been induced to differentiate to an artificial tissue structure such as artificial neural tissue, said medium comp...

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Bibliographic Details
Main Authors BOSIO, ANDREAS, AGORKU, DAVID JOEL, KNOBEL, SEBASTIAN, ECKARDT, DOMINIK, HARDT, OLAF THORSTEN, BECKER, KRISTIN
Format Patent
LanguageEnglish
French
Published 20.08.2020
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:The present invention provides a differentiation medium for differentiation and expansion of a multicellular aggregation in suspension derived from human pluripotent stem cells that has been induced to differentiate to an artificial tissue structure such as artificial neural tissue, said medium comprising a basal medium for animal or human cells, wherein said differentiation medium has a viscosity between 1.7 mPa*s and 1500 mPa*s. Said viscosity is achieved by the presence of a viscosity enhancer such as methyl cellulose, carboxymethyl cellulose, or hydroxy ethyl cellulose in said differentiation medium. Also disclosed are an in-vitro method for obtaining artificial neural tissue and a kit comprising said differentiation medium. La présente invention concerne un milieu de différenciation pour la différenciation et l'expansion d'une agrégation multicellulaire en suspension dérivée de cellules souches pluripotentes humaines qui ont été induites pour se différencier en une structure de tissu artificiel telle qu'un tissu neuronal artificiel, ledit milieu comprenant un milieu de base pour des cellules animales ou humaines, ledit milieu de différenciation ayant une viscosité comprise entre 1,7 mPa*s et 1 500 mPa*s Ladite viscosité est obtenue par la présence d'un activateur de viscosité tel que la méthylcellulose, la carboxyméthylcellulose ou l'hydroxyéthyl cellulose dans ledit milieu de différenciation. L'invention concerne également un procédé in-vitro pour obtenir un tissu neuronal artificiel et un kit comprenant ledit milieu de différenciation.
Bibliography:Application Number: CA20203129656