PROMOTERS FROM CORYNEBACTERIUM GLUTAMICUM AND USES THEREOF IN REGULATING ANCILLARY GENE EXPRESSION

Provided are native promoters comprising polynucleotides isolated from Corynebacterium glutamicum, and mutant promoters derived therefrom, which may be used to regulate, i.e., either increase or decrease, on-pathway and/or off-pathway gene expression. Also provided are promoter ladders comprising a...

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Bibliographic Details
Main Authors GORA, KATHERINE G, SHEARER, ALEXANDER, ENYEART, PETER, SERBER, ZACH, MANCHESTER, SHAWN P
Format Patent
LanguageEnglish
French
Published 13.12.2018
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:Provided are native promoters comprising polynucleotides isolated from Corynebacterium glutamicum, and mutant promoters derived therefrom, which may be used to regulate, i.e., either increase or decrease, on-pathway and/or off-pathway gene expression. Also provided are promoter ladders comprising a plurality of the promoters having incrementally increasing promoter activity. Also provided are host cells and recombinant vectors comprising the promoters, and methods of expressing ancillary genes of interest and producing biomolecules using the host cells. La présente invention concerne des promoteurs natifs comprenant des polynucléotides isolés de Corynebacterium glutamicum, ainsi que des promoteurs mutants qui sont dérivés de ces derniers et qui peuvent servir à réguler, c'est-à-dire augmenter ou réduire, l'expression génique dans la voie et/ou hors voie. L'invention concerne également des échelles de promoteurs comprenant une pluralité de promoteurs ayant une activité de promoteur augmentant progressivement. L'invention concerne en outre des cellules hôtes et des vecteurs recombinants comprenant lesdits promoteurs, ainsi que des procédés d'expression des gènes ancillaires d'intérêt et de production de biomolécules au moyen des cellules hôtes.
Bibliography:Application Number: CA20183064777