METHOD OF PRODUCING RECOMBINANT SUCROSE SYNTHASE, USE THEREOF IN THE PRODUCTION OF SUCROSE-DETERMINATION KITS, METHOD OF PRODUCING ADPGLUCOSE AND METHOD OF OBTAINING TRANSGENIC PLANTS HAVING LEAVES AND RESERVE ORGANS WHICH ACCUMULATE A HIGH CONCENTRATION OF ADPGLUCOSE AND STARCH

• Main Authors: MORAN ZORZANO, MARIA TERESA, ALONSO CASAJUS, NORA, MUNOZ PEREZ, FRANCISCO JOSE, BAROJA FERNANDEZ, MIREN EDURNE, POZUETA ROMERO, FRANCISCO JAVIER
• Format: Patent
• Language: English; French
• Published: 15.10.2019
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRAPPING; VINEGAR; WINE

It is provided a method for the efficient production of large quantities of soluble recombinant sucrose synthase (SS) in the active form thereof, with the expression of the gene that codes for SS in a strain Of Escherichia coll. The expression vector used enables the recombinant SS thus produced to have a histidine tail which facilitates the rapid purification thereof. In addition, it is provided sequences of mutated versions of the SS gene, which code for SS isoforms that are suitable for the production of ADPG. It is also provided a method of obtaining transgenic plants which overexpress the SS gene, either constituently or in the reserve organs or leaves thereof, and which have a high concentration (both in the leaves and in the reserve tissues) of sucrose, ADPG, G6P and starch owing to the high ADPG-synthesising activity of SS. Un procédé de production efficace de grandes quantités de SS recombinante soluble sous sa forme active au moyen de lexpression du gène codant pour la SS dans une souche de Escherichia coli. Le vecteur dexpression utilisé permet que la SS recombinante obtenue possède une queue dhistidines facilitant sa purification de manière rapide. Sont également décrites les séquences de version mutées du gène de la SS codant pour des isoformes de la SS adéquates pour la production dADPG. Finalement, on décrit la description de plantes transgéniques qui surexpriment le gène de la SS, soit par constitution, soit sous forme de feuilles ou organes de réserve, et qui possèdent une forte teneur (en feuilles et en tissus de réserve) en saccharose, ADPG, G6P et amidon comme résultat de la forte activité de synthèse dADPG de la SS.