METHODS OF CONJUGATING AN AGENT TO A THIOL MOIETY IN A PROTEIN THAT CONTAINS AT LEAST ONE TRISULFIDE BOND

The invention provides improved methods of conjugating an agent to a thiol moiety in a protein that contains at least one disulfide bond and at least one trisulfide bond. Exemplary embodiments include the production of antibody drug conjugates substantially free of impurities created in the presence...

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Bibliographic Details
Main Authors BECHTEL, CHARITY TUCKER, TULLY, TIMOTHY, FRANKLIN, JAYME, HUTCHINSON, MATTHEW, LIN, XIN XIN, GORRELL, JEFFREY
Format Patent
LanguageEnglish
French
Published 11.04.2023
Subjects
CHEMISTRY
HUMAN NECESSITIES
HYGIENE
MEDICAL OR VETERINARY SCIENCE
METALLURGY
ORGANIC CHEMISTRY
PEPTIDES
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
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Summary:The invention provides improved methods of conjugating an agent to a thiol moiety in a protein that contains at least one disulfide bond and at least one trisulfide bond. Exemplary embodiments include the production of antibody drug conjugates substantially free of impurities created in the presence of reactive sulfide moieties in the production processes. L'invention concerne des procédés améliorés de conjugaison d'un agent à une fraction thiol dans une protéine qui contient au moins une liaison disulfure et au moins une liaison trisulfure. Des modes de réalisation pour exemple comprennent la production de conjugués anticorps-médicament pratiquement exempts d'impuretés, élaborés en présence de fractions sulfure réactives dans les processus de production.
Bibliography:Application Number: CA20152955007