COMPOSITIONS AND METHODS FOR STABILIZING LUMINOGENIC SUBSTRATES

• Main Authors: KIRKLAND, THOMAS, WOOD, KEITH V, HALL, MARY, SCHWINN, MARIE
• Format: Patent
• Language: English; French
• Published: 26.11.2015
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; HETEROCYCLIC COMPOUNDS; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; TESTING; VINEGAR; WINE

Disclosed herein are compositions and methods for stabilizing a luminogenic substrate such as coelenterazine or a functional analog thereof. The functional analog may be furimazine. The composition may include the luminogenic substrate, a thionucleoside, and an organic solvent, in which the thionucleoside is present in an amount effective to stabilize the luminogenic composition against decomposition. The method provided herein stabilizes the luminogenic substrate against decomposition by contacting the luminogenic substrate with an effective amount of the thionucleoside in the presence of the organic solvent. Also provided herein is a kit containing the composition. L'invention concerne des compositions et des procédés de stabilisation d'un substrat luminogène tel que la coelenterazine ou un analogue fonctionnel de celle-ci. L'analogue fonctionnel peut être la furimazine. La composition peut comprendre le substrat luminogène, un thionucléoside, et un solvant organique, le thionucléoside étant présent en une quantité efficace pour stabiliser la composition luminogène contre la décomposition. Le procédé de la présente invention stabilise le substrat luminogène contre la décomposition par la mise en contact du substrat luminogène avec une quantité efficace du thionucléoside en la présence du solvant organique. L'invention concerne également une trousse contenant la composition.