LIGASE-ASSISTED NUCLEIC ACID CIRCULARIZATION AND AMPLIFICATION

• Main Authors: NELSON, JOHN RICHARD, KVAM, ERIK LEEMING, HELLER, RYAN CHARLES
• Format: Patent
• Language: English; French
• Published: 06.12.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

Provided herein are methods for generation and amplification of a single-stranded DNA circle in a single reaction vessel from a linear DNA without any intervening purification steps. The single-stranded DNA circle is generated via a template-independent single-stranded DNA ligation. Whole-genome amplification of circulating nucleic acids extracted from blood is provided. Kits for performing the disclosed methods are also provided. L'invention concerne des méthodes de production et d'amplification d'un cercle d'ADN monocaténaire dans un réacteur unique à partir d'un ADN linéaire, sans aucune étape de purification. Le cercle d'ADN monocaténaire est produit par l'intermédiaire d'une ligature d'ADN monocaténaire indépendant de la matrice. L'invention concerne également l'amplification complète du génome d'acides nucléiques circulants extraits du sang. L'invention concerne encore des kits permettant la mise en oeuvre de ces méthodes.