WHOLE-CELL SYSTEM FOR CYTOCHROME P450 MONOOXYGENASES BIOCATALYSIS

• Main Authors: KLESER, MICHAEL, BERNHARDT, RITA, GERBER, ADRIAN, HANNEMANN, FRANK, BLEIF, SABRINA
• Format: Patent
• Language: English; French
• Published: 24.12.2014
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The subject of the present invention is a whole-cell catalysis process for converting substrates of cytochrome P450 monooxygenases of eukaryotic origin into valuable biotechnological products. The subject of the present invention is also microorganisms genetically engineered to achieve those biotransformations with high rates and processes to prepare these microorganism strains. La présente invention concerne un procédé de catalyse à cellules entières pour la conversion de substrats de monooxygénases à cytochromes P450 d'origine eucaryote en produits biotechnologiques de valeur. La présente invention concerne également des microorganismes génétiquement modifiés pour permettre ces biotransformations à des taux élevés et des procédés de préparation de ces souches de microorganisme.