A METHOD OF PRODUCING TRANSGENIC CORN PLANTS FROM CALLUS OF CORN EMBRYOS

• Main Authors: WILKS, JOEL B, ROUT, JYOTI R, DUNCAN, DAVID R, PETERSEN, WILLIAM L, MANN, MICHAEL T, LOWE, BRENDA, ZHANG, WANGGEN, AKULA, ANISHA, SONGSTAD, DAVID D
• Format: Patent
• Language: English; French
• Published: 29.08.2017
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRAPPING; VINEGAR; WINE

A method for producing a transformable and embryogenic corn cell culture comprising: a) obtaining a Type II callus from corn embryos; b) initiating a cell suspension culture from cells of the callus; and c) culturing the cell suspension for about 5 days to 30 days in a liquid medium to produce the cell culture, wherein the cell culture is transferred to fresh growth media every 2-3 days; and wherein undesirable cell types are removed from the cell culture during transfer to fresh growth media. Procédé de production dune culture de cellule de maïs transformable et embryogène comprenant ceci : a) obtenir un callus de type II à partir dembryons de maïs; b) démarrer une culture en suspension cellulaire à partir des cellules du callus; et c) cultiver la suspension cellulaire pendant environ cinq à 30 jours, dans un milieu liquide, pour produire la culture cellulaire, ladite culture cellulaire pouvant être transférée dans un milieu de croissance frais tous les deux à trois jours. Selon linvention, les types de cellules indésirables sont retirés de la culture cellulaire pendant le transfert vers le milieu de croissance frais.