A METHOD FOR FILTERING A BIOLOGICAL SAMPLE

A method for filtering a sample comprising the steps of: (a) providing a sample with eukaryotic cells and containing or suspected to contain a micro-organism; (b) performing a selective lysis of the eukaryotic cells to obtain a lysed sample; (c) filtering the lysed sample obtained in step (b) throug...

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Bibliographic Details
Main Authors PENTERMAN, ROEL, VAN MEERBERGEN, BART
Format Patent
LanguageEnglish
French
Published 16.01.2014
Subjects
APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
COMPOSITIONS THEREOF
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
CULTURE MEDIA
ENZYMOLOGY
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROCESSES OF PREPARING SUCH COMPOSITIONS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:A method for filtering a sample comprising the steps of: (a) providing a sample with eukaryotic cells and containing or suspected to contain a micro-organism; (b) performing a selective lysis of the eukaryotic cells to obtain a lysed sample; (c) filtering the lysed sample obtained in step (b) through a filter arranged to retain the micro-organism; (d) washing the filter with a detergent based wash buffer to selectively solubilize proteins originating from the eukaryotic cells retained by the filter, by passing the detergent based wash buffer through the filter, to remove protein clogs from the filter in order to allow an additional step (c) of filtering said lysed sample.
Bibliography:Application Number: CA20132871212