METHOD FOR THE IDENTIFICATION OF T CELL EPITOPES

A novel method to identify relevant T-cell epitopes recognized by CD8+ or CD4+ T lymphocytes is described. The method is based on the use of mRNA fragments synthesized from cDNA encoding portions of a polypeptide of interest. mRNA fragments are introduced into antigen-presenting cells to deduce an e...

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Bibliographic Details
Main Authors DOUCET, JEAN-DANIEL, LAPOINTE, REJEAN
Format Patent
LanguageEnglish
French
Published 29.10.2019
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
COMPOSITIONS THEREOF
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
CULTURE MEDIA
ENZYMOLOGY
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
ORGANIC CHEMISTRY
PEPTIDES
PROCESSES OF PREPARING SUCH COMPOSITIONS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:A novel method to identify relevant T-cell epitopes recognized by CD8+ or CD4+ T lymphocytes is described. The method is based on the use of mRNA fragments synthesized from cDNA encoding portions of a polypeptide of interest. mRNA fragments are introduced into antigen-presenting cells to deduce an epitope's localization in a polypeptide of interest, such as a protein antigen. La présente invention concerne une nouvelle méthode d'identification d'épitopes de cellules T d'intérêt reconnus par les lymphocytes T CD8+ ou CD4+. La méthode est basée sur l'emploi de fragments d'ARNm synthétisés à partir d'ADNc codant des portions d'un polypeptide d'intérêt. Les fragments d'ARNm sont introduits dans des cellules présentant l'antigène pour déduire la localisation d'un épitope dans un polypeptide d'intérêt, tel qu'un antigène protéinique.
Bibliography:Application Number: CA20112834001