MEMBRANE BOUND REPORTER MOLECULES AND THEIR USE IN CELL SORTING

• Main Authors: HELMAN, DANIEL, BAR-SHIMON, MEIRAV, SMOLARSKY, MOSHE, TOISTER-ACHITUV, MIRA
• Format: Patent
• Language: English; French
• Published: 08.01.2019
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PHYSICS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TESTING; VINEGAR; WINE

The present invention relates to nucleic acid molecules comprising a nucleic acid sequence encoding a membrane-bound biotin mimetic peptide (BMP) or biotin acceptor peptide (BAP). The invention also relates to a method for selection of high producer cells secreting a protein of interest. La présente invention concerne des molécules d'acide nucléique comprenant une séquence d'acide nucléique codant pour un peptide mimétique de la biotine lié à la membrane (BMP) ou un peptide accepteur de biotine (BAP). L'invention concerne en outre un procédé de sélection de cellules grandes productrices sécrétant une protéine d'intérêt.