RNA SYNTHESIS - PHOSPHORAMIDITES FOR SYNTHETIC RNA IN THE REVERSE DIRECTION, AND APPLICATION IN CONVENIENT INTRODUCTION OF LIGANDS, CHROMOPHORES AND MODIFICATIONS OF SYNTHETIC RNA AT THE 3' - END
The present invention relates to novel phosphoramidites, A-n-bz, C-n-bz, C-n-ac, G-n-ac and U are produced with an HPLC purity of greater than 98% and 31P NMR purity greater than 99%. A novel process of reverse 5'.fwdarw. 3' directed synthesis of RNA oligomers has been developed and disclo...
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Main Authors | , , , |
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Format | Patent |
Language | English French |
Published |
11.07.2017
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Subjects | |
Online Access | Get full text |
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Summary: | The present invention relates to novel phosphoramidites, A-n-bz, C-n-bz, C-n-ac, G-n-ac and U are produced with an HPLC purity of greater than 98% and 31P NMR purity greater than 99%. A novel process of reverse 5'.fwdarw. 3' directed synthesis of RNA oligomers has been developed and disclosed. Using that method demonstrated high quality RNA synthesis with coupling efficiency approaching 99%.
La présente invention concerne de nouveaux phosphoramidites, A-n-bz, C-n-bz, C-n-ac, G-n-ac et U qui sont produits avec une pureté par CLHP supérieure à 98 % et une pureté par RMN au 31P supérieure à 99 %. Un nouveau procédé de synthèse antisens dirigée de 5' ? 3' doligomères dARN a été développé et présenté. Lutilisation de ce procédé a montré une qualité de synthèse dARN élevée avec une efficacité de couplage approchant les 99 %. |
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Bibliography: | Application Number: CA20092735251 |