OPTIMISATION OF EXPRESSION OF PARVOVIRAL REP AND CAP PROTEINS IN INSECT CELLS

• Main Authors: HERMENS, WILHELMUS THEODORUS JOHANNES MARIA CHRISTIAAN, BAKKER, ANDREW CHRISTIAN, NOORDMAN, YVET
• Format: Patent
• Language: English; French
• Published: 12.01.2021
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The present invention relates to the improved production of recombinant parvoviral virions in insect cells. In par-ticular, the invention relates to an improved process for the production of recombinant parvoviral virions in insect cells, wherein the full/empty parvoviral virion ratio is increased. The invention also relates to the production of parvoviral vectors that may be used in gene therapy and to improvements in expression of the viral Rep proteins that increase the productivity of parvoviral vec-tors. L'invention concerne la production améliorée de virions parvoviraux recombinants dans des cellules d'insectes. L'invention concerne en outre un procédé amélioré de production de virions parvoviraux recombinants dans des cellules d'insectes, le rapport plein/vide de virions parvoviraux étant accru. L'invention concerne également la production de vecteurs parvoviraux que l'on peut utiliser en thérapie génique ainsi que des améliorations en expression de protéines Rep virales qui augmentent la productivité des vecteurs parvoviraux.