IDENTIFICATION OF TRPML3 (MCOLN3) AS A SALTY TASTE RECEPTOR AND USE IN ASSAYS FOR IDENTIFYING TASTE (SALTY) MODULATORS AND/OR THERAPEUTICS THAT MODULATE SODIUM TRANSPORT, ABSORPTION OR EXCRETION AND/OR ALDOSTERONE AND/OR VASOPRESSIN PRODUCTION OR RELEASE

• Main Authors: ZLOTNIK, ALBERT, HEVEZI, PETER, WILLIAMS, MARK, DAHAN, DAVID, SERVANT, GUY, KALABAT, DALIA, BRUST, PAUL, SAGANICH, MICHAEL, LU, MIN, SOTO, HORTENSIA, GAO, NA, LAITA, BIANCA, WHITE, EVAN CARL, MOYER, BRYAN
• Format: Patent
• Language: English; French
• Published: 15.01.2009
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMBINATORIAL CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICOLIBRARIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TESTING; VINEGAR; WINE

High-throughput mammalian and medium-throughput oocyte-based electrophysi ological assays for identifying human TRPML3 modulators, preferably TRPML3 e nhancers are disclosed Compounds that modulate TRPML3 function in the assay are expected to affect salty taste in humans The electrophysiological assays , such as the two-electrode voltage-clamp technique, facilitate the identifi cation of compounds which specifically modulate human TRPML3 The assays prov ide a robust screen useful to detect compounds that facilitate (enhance) or inhibit TRPML3 function and thereby modulate salty taste In addition, these compounds may be used to regulate sodium excretion, urinary output and other biological functions relating to sodium levels