DIAGNOSTIC TEST FOR THE DETECTION OF A MOLECULE OR DRUG IN WHOLE BLOOD

• Main Authors: GRENIER, FRANK C, SYED, HINA N, ALI, SALMAN, WORKMAN, RYAN F
• Format: Patent
• Language: English; French
• Published: 23.06.2015
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; TESTING; VINEGAR; WINE

The invention provides methods of preparing a test sample for use in an assay for detecting an analyte bound by an intracellular ligand. The methods typically involve contacting the test sample with an assay reagent comprising: a lysis reagent; and a protease that has proteolytic activity for said intracellular ligand; to form a mixture compatible for use in an immunoassay without subsequent extraction steps. Other aspects of the invention include related immunoassays and test kits. Procédé de préparation d'un échantillon d'essai pour une analyse visant à détecter une substance liée à un ligand intracellulaire. De façon générale, le procédé consiste à mettre l'échantillon d'essai en contact avec un réactif comprenant: un réactif liquide; et une protéase à activité protéolytique pour ledit ligand intracellulaire, le but étant de former un mélange compatible pour utilisation dans une analyse immunologique sans opération d'extraction ultérieures. Autres aspects de l'invention: analyses immunologiques et des trousses d'essai connexes.