PRODUCTION OF HIGH TRYPTOPHAN MAIZE BY CHLOROPLAST TARGETED EXPRESSION OF ANTHRANILATE SYNTHASE

• Main Authors: RAPP, WILLIAM D, VARAGONA, MARGUERITE J, WINSON, JENNIFER L, NAVARRO, SANTIAGO XAVIER, YE, GUANGNING, SHI, XIAOHONG, MANJUNATH, SIVA
• Format: Patent
• Language: English; French
• Published: 16.05.2017
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FOODS OR FOODSTUFFS; FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BYSUBCLASSES A23B - A23J; FORESTRY; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; ORGANIC CHEMISTRY; PEPTIDES; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OFNUTRITIVE QUALITIES, PHYSICAL TREATMENT; THEIR TREATMENT, NOT COVERED BY OTHER CLASSES; TRAPPING; VINEGAR; WINE

Novel expression vectors and constructs encoding a chloroplast transit peptide (CTP) operably linked to a monomeric anthranilate synthase are provided. Additionally, novel polynucleotide sequences encoding monomeric anthranilate synthases are provided. Also provided are methods for increasing the levels of free tryptophan in transgenic plants containing the expression vectors and constructs. La présente invention concerne de nouveaux vecteurs d'expression et des constructions codant pour un peptide de transit de chloroplaste (PTC) lié de manière fonctionnelle à une anthranilate synthase monomère. L'invention concerne de plus de nouvelles séquences polynucléotidiques codant pour des anthranilate synthases monomères. L'invention concerne également des procédés permettant d'accroître les niveaux de tryptophane libre dans des plantes transgéniques contenant les vecteurs d'expression et les constructions.