ACYL-COA-DEPENDENT DIACYLGLYCEROL ACYLTRANSFERASE 1 (DGAT1 ) GENE FROM TROPAEOLUM MAJUS, PROTEIN ENCODED THEREBY AND USES THEREOF

• Main Authors: TAYLOR, DAVID C, MIETKIEWSKA, ELZBIETA, FRANCIS, TAMMY, XU, JINGYU
• Format: Patent
• Language: English; French
• Published: 10.02.2015
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRAPPING; VINEGAR; WINE

A diacylglycerol acyltransferase (DGAT1) gene from Tropaeolum majus encodes a DGAT1 polypeptide that regulates the production of triacylglycerols from diacylglycerols. Transformation of cells with the Tropaeolum majus DGAT1 (TmDGATI) gene, or variants thereof, leads to changes in DGATI activity and alterations in oil and/or fatty acid content and/or seed weight. In particular, oil content, seed weight, erucic acid and/or trierucin content may be increased. La présente invention concerne un gène de diacylglycérol acyltransférase (DGAT1) de capucine qui code un polypeptide DGAT1 régulant la production de triacylglycérols à partir de diacylglycérols. La transformation de cellules présentant le gène DGAT1 de capucine (TmDGAT1) ou de variantes de celles-ci conduit à des modifications de l'activité de DGAT1 et à des variations de la teneur en huile et/ou en acides gras et/ou du poids des graines. Il est notamment possible d'augmenter la teneur en huile, le poids des graines et la teneur en acide érucique et/ou en triérucine.