PCR ELBOW DETERMINATION USING QUADRATIC TEST FOR CURVATURE ANALYSIS OF A DOUBLE SIGMOID

• Main Authors: KURNIK, RONALD T, ADITYA, SANE
• Format: Patent
• Language: English; French
• Published: 25.03.2009
• Subjects: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; BEER; BIOCHEMISTRY; CALCULATING; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPUTING; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; COUNTING; ELECTRIC DIGITAL DATA PROCESSING; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

Systems and methods for determining whether the data for a growth curve represents or exhibits valid or significant growth. A data set representing a sigmoid or growth-type curve, such as a PCR curve, is processed to determine whether the data exhibits significant or valid growth. A first or a second degree polynomial curve that fits the data is determined, and a statistical significance value for t he curve fit is determined. If the significance value exceeds a significance threshold, the data is considered to not represent significant or valid growth. If the data does no t represent significant or valid growth, the data set may be discarded. If the significance value does not exceed the significance threshold, the data is considered to represent significant or valid growth. If the data set is determined to represent vali d growth, the data is further processed to determine a transition value in the sigmoid or growth curve, such as the end of the baseline region or the elbow value or Ct value of a PCR amplification curve.