METHODS AND COMPOSITIONS FOR A MICROEMULSION-BASED TISSUE TREATMENT

• Main Authors: KRAM, BRIAN, DRUMHELLER, PAUL D, BIENIARZ, CHRISTOPHER
• Format: Patent
• Language: English; French
• Published: 27.03.2012
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MIXING, e.g. DISSOLVING, EMULSIFYING, DISPERSING; PERFORMING OPERATIONS; PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL; PHYSICS; TESTING; TRANSPORTING

The invention is directed to methods and compositions for deparaffinizing paraffin-embedded biological samples for subsequent tissue staining. The compositions are microemulsions that may include water/oil/surfactant microemulsions, and optionally a cosurfactant. The microemulsions enable deparaffinization without the use of xylene or toluene, and also enable solvent exchange without the use of intermediary alcohol dehydration or alcohol rehydration compositions. L'invention porte sur des procédés et des compositions permettant de procéder au déparaffinage d'échantillons biologiques incrustés dans la paraffine en vue d'une coloration ultérieure de tissus. Ces compositions sont des micro-émulsions pouvant contenir des micro-émulsions d'eau/huile/agent de surface, et facultativement un cotensioactif. Ces micro-émulsions permettent le déparaffinage sans utiliser du xylène ou du toluène, et permettent aussi l'échange de solvants sans utiliser des compositions intermédiaires de déshydratation d'alcool ou de réhydratation d'alcool.