INSTRUMENT, METHOD AND KIT FOR DETECTING LACTOBACILLUS BREVIS AND LACTOBACILLUS LINDNERI

• Main Authors: NAKAGAWA, HIROKO, OSANO, KAORU, MORIYA, SHOUGO, KODAMA, YUKIKO, HATANAKA, KAZUSHIGE, TANAKA, KOICHI
• Format: Patent
• Language: English; French
• Published: 08.01.2013
• Subjects: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The present invention provides an instrument for detecting Lactobacillus brevis and Lactobacillus lindneri by which bacteria contaminating a test sample can be quickly and exactly determined by a convenient procedure, a method of detecting Lactobacillus brevis and Lactobacillus lindneri and a kit for detecting Lactobacillus brevis and Lactobacillus lindneri. In particular, the present invention provides a microarray type instrument wherein an oligonucleotide based on a nucleotide sequence specific to the genus or species of Lactobacillus brevis and Lactobacillus lindneri, to which the target bacteria belong, is immobilized on the surface of a substrate. The target bacteria in the test sample can be conveniently, quickly and exactly detected and identified depending on the occurance or non-occurance of the hybridization between a probe prepared from the test sample and the oligonucleotide immobilized on the surface of the substrate.