METHOD FOR TRANSDIFFERENTIATION OF NON-PANCREATIC STEM CELLSTO THE PANCREATIC PATHWAY

• Main Author: RAMIYA, VIJAYAKUMAR K
• Format: Patent
• Language: English; French
• Published: 14.10.2004
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The subject invention relates to a method of transdifferentiating mammalian non-pancreatic stem cells, such as mesenchymal stem cells (MSCs), to enter t he pancreatic differentiations pathway. The MSCs are cultured under conditions that permit the expression of pancreatic differentiation markers, and these conditions include use of: (a) culture conditions that promote cell clustering; and/or (b) medium comprising glucagon-like peptide-1(GLP-1), hepatocyte growth factor (HGF) and/or nicotinamide.