QUANTITATION OF BIOLOGICAL MOLECULES

Methods and apparatus, including computer program products, for quantifying peptides in a peptide mixture. A peptide mixture containing a plurality of peptides is received. One or more peptides are separated from the peptide mixture over a period of time. One or more of the peptides separated at a p...

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Bibliographic Details
Main Authors SHALTER, THOMAS A, CHELIUS, DIRK H, BONDAREKO, PAVEL V
Format Patent
LanguageEnglish
French
Published 30.10.2003
Edition7
Subjects
CHEMISTRY
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
METALLURGY
ORGANIC CHEMISTRY
PEPTIDES
PHYSICS
TESTING
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Summary:Methods and apparatus, including computer program products, for quantifying peptides in a peptide mixture. A peptide mixture containing a plurality of peptides is received. One or more peptides are separated from the peptide mixture over a period of time. One or more of the peptides separated at a particular time are subjected to mass-to-charge analysis and an abundance of one or more of the mass analyzed peptides is calculated. A relative quantity for the one or more mass analyzed peptides is calculated by comparing the calculated abundance of the peptides with an abundance of one or more peptid es in a reference sample that is external to the first peptide mixture. The techniques can be applied to arbitrary peptides, without requiring the use o f differential mass labeling, and can be applied to other biological molecules , such as nucleic acids and small molecules.
Bibliography:Application Number: CA20032484078