ZYMOMONAS PENTOSE-SUGAR FERMENTING STRAINS AND USES THEREOF

A Zymomonas integrant posseses the ability to ferment pentose into ethanol. The operon encoding for the pentose-fermenting enzymes are integrated into t he Zymomonas genome in a two-integration event of homologous recombination and transposition. Each operon may include more than one pentose-reducin...

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Bibliographic Details
Main Authors HOWE, WILLIAM, EVANS, KENT, EDDY, CHRISTINE, ZHANG, MIN, CHOU, YATN
Format Patent
LanguageEnglish
French
Published 06.05.2004
Edition7
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:A Zymomonas integrant posseses the ability to ferment pentose into ethanol. The operon encoding for the pentose-fermenting enzymes are integrated into t he Zymomonas genome in a two-integration event of homologous recombination and transposition. Each operon may include more than one pentose-reducing enzyme encoding sequence. The integrant in some embodiments includes enzyme sequenc es encoding xylose isomerase, xylulokinase, transketolase and transaldolase. Th e Zymomonas integrants are highly stable, and retain activity for producing th e pentose-fermenting enzyme for between 80 to 160 generations. The integrants are also resistant to acetate inhibition, as the integrants demonstrate efficient ethanol production even in the presence of 8 up to 16 grams acetat e per liter media.
Bibliography:Application Number: CA20032483574