ZYMOMONAS PENTOSE-SUGAR FERMENTING STRAINS AND USES THEREOF
A Zymomonas integrant posseses the ability to ferment pentose into ethanol. The operon encoding for the pentose-fermenting enzymes are integrated into t he Zymomonas genome in a two-integration event of homologous recombination and transposition. Each operon may include more than one pentose-reducin...
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Main Authors | , , , , |
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Format | Patent |
Language | English French |
Published |
06.05.2004
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Edition | 7 |
Subjects | |
Online Access | Get full text |
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Summary: | A Zymomonas integrant posseses the ability to ferment pentose into ethanol. The operon encoding for the pentose-fermenting enzymes are integrated into t he Zymomonas genome in a two-integration event of homologous recombination and transposition. Each operon may include more than one pentose-reducing enzyme encoding sequence. The integrant in some embodiments includes enzyme sequenc es encoding xylose isomerase, xylulokinase, transketolase and transaldolase. Th e Zymomonas integrants are highly stable, and retain activity for producing th e pentose-fermenting enzyme for between 80 to 160 generations. The integrants are also resistant to acetate inhibition, as the integrants demonstrate efficient ethanol production even in the presence of 8 up to 16 grams acetat e per liter media. |
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Bibliography: | Application Number: CA20032483574 |