PROCESS FOR PREPARING OLIGONUCLEOTIDES

• Main Authors: MOODY, DAVID JOHN, WELLINGS, DONALD ALFRED, MCCORMAC, PAUL
• Format: Patent
• Language: English; French
• Published: 09.04.2013
• Subjects: ACYCLIC OR CARBOCYCLIC COMPOUNDS; BEER; BIOCHEMISTRY; CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOIDCHEMISTRY; CHEMISTRY; COMPOSITIONS BASED THEREON; COMPOSITIONS OF MACROMOLECULAR COMPOUNDS; COMPOSITIONS THEREOF; CULTURE MEDIA; DERIVATIVES THEREOF; ENZYMOLOGY; MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVINGCARBON-TO-CARBON UNSATURATED BONDS; MACROMOLECULAR COMPOUNDS OBTAINED OTHERWISE THAN BY REACTIONSONLY INVOLVING UNSATURATED CARBON-TO-CARBON BONDS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NUCLEIC ACIDS; NUCLEOSIDES; NUCLEOTIDES; ORGANIC CHEMISTRY; ORGANIC MACROMOLECULAR COMPOUNDS; PERFORMING OPERATIONS; PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; SUGARS; THEIR PREPARATION OR CHEMICAL WORKING-UP; THEIR RELEVANT APPARATUS; TRANSPORTING; VINEGAR; WINE

A process for the preparation of an oligonucleotide is provided. The process comprises the assembly of an oligonucleotide attached to a solid support, wherein the solid support is prepared by a process comprising polymerisation of a monomer which comprises a protected hydroxypolyC2-4 alkyleneoxy chain attached to a polymerisable unit wherein the protected hydroxypolyC2-4alkyleneoxy chain contains from 2 to 10 C2-4 alkyleneoxy groups and wherein the hydroxypolyC2-4 alkyleneoxy chain is protected with an acid-labile protecting group, preferably an optionally substituted trityl group. Procédé de préparation d'un oligonucléotide reposant sur l'utilisation d'un ensemble constitué d'un oligonucléotide attaché à un support solide. Ledit support solide est préparé selon un procédé consistant à polymériser un monomère qui possède une chaîne hydroxypolyC¿2-4 ?alkylèneoxy protégée attachée à une unité polymérisable. La chaîne hydroxypolyC¿2-4 ?alkylèneoxy protégée, qui contient 2 à 10 groupes alkylèneoxy C¿2-4?, est protégée à l'aide d'une groupe protecteur labile sous l'effet d'un acide, de préférence un groupe trityle éventuellement substitué.