METHOD OF CONSTRUCTING CDNA TAG FOR IDENTIFYING EXPRESSED GENE AND METHOD OF ANALYZING GENE EXPRESSION

A cDNA tag for identifying an expressed gene is constructed by combining two types of linkers X and Y containing a II type restriction enzyme, two IIS ty pe restriction enzymes and the recognition sequence of one of the IIS type restriction enzymes. This cDNA tag is usable in analyzing an expressed...

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Bibliographic Details
Main Authors KASAI, JUN, YAMAMOTO, MIKIO, YAMAMOTO, NAOKI, HIROSE, KUNITAKA
Format Patent
LanguageEnglish
French
Published 26.09.2002
Edition7
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
ENZYMOLOGY
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
PROCESSES OF PREPARING SUCH COMPOSITIONS
SPIRITS
VINEGAR
WINE
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Summary:A cDNA tag for identifying an expressed gene is constructed by combining two types of linkers X and Y containing a II type restriction enzyme, two IIS ty pe restriction enzymes and the recognition sequence of one of the IIS type restriction enzymes. This cDNA tag is usable in analyzing an expressed gene either as such or after a ligation treatment or the like.
Bibliography:Application Number: CA20022455354