METHODS OF PROTEIN PURIFICATION AND RECOVERY

Improved methods for purification and recovery of interferon-beta (IFN-.beta.) and compositions comprising substantially monomeric IFN-.beta. are provided. In one purification method, substantially purified IFN-.beta. or variant thereof is precipitated and then dissolved in a guanidine hydrochloride...

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Bibliographic Details
Main Authors BABUKA, SUSAN, WOLFE, SID, SHIRLEY, BRET, A, ESIKOVA, IRINA, FORDHAM, DENNIS
Format Patent
LanguageEnglish
French
Published 02.05.2002
Edition7
Subjects
CHEMISTRY
HUMAN NECESSITIES
HYGIENE
MEDICAL OR VETERINARY SCIENCE
METALLURGY
ORGANIC CHEMISTRY
PEPTIDES
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
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Summary:Improved methods for purification and recovery of interferon-beta (IFN-.beta.) and compositions comprising substantially monomeric IFN-.beta. are provided. In one purification method, substantially purified IFN-.beta. or variant thereof is precipitated and then dissolved in a guanidine hydrochloride (HC1) solution. Renaturation of the protein occurs by dilution with a suitable buffer. A similar purification method absent the precipitation step is also provided. Following renaturation of the IFN-.beta., residual guanidine HC1 is removed by diafiltration or dialysis with a pharmaceutically acceptable buffer to prepare pharmaceutical compositions comprising substantially monomeric IFN- .beta..
Bibliography:Application Number: CA20012427088