SOLID SUPPORT ASSAY SYSTEMS AND METHODS UTILIZING NON-STANDARD BASES
Solid support assays using non-standard bases are described. A capture oligonucleotide comprising a molecular recognition sequence is attached to a solid support and hybridized with a target oligonucleotide the solid support. In some instances, the molecular recognition sequence includes one or more...
Saved in:
Main Authors | , , , , , , , |
---|---|
Format | Patent |
Language | English French |
Published |
24.01.2012
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | Solid support assays using non-standard bases are described. A capture oligonucleotide comprising a molecular recognition sequence is attached to a solid support and hybridized with a target oligonucleotide the solid support. In some instances, the molecular recognition sequence includes one or more non-standard bases and hybridizes to a complementary tagging sequence of the target oligonucleotide. In other instances, incorpoaration of a non-standard base (e.g., via PCR or ligation) is used in the assay. |
---|---|
Bibliography: | Application Number: CA20012425747 |