IMPROVED METHODS FOR GROWING MAMMALIAN CELLS IN VITRO

The invention relates to a method for reduction of glucose consumption and/or lactate production during cultivation of animal cells, characterized in that cultivation is performed in the presence of a bi- or tricarbonic acid or a salt thereof at a concentration of about 1 to 580 mmol/l, whereby in t...

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Bibliographic Details
Main Authors EBERHARDT, HORST, SZPERALSKI, BERTHOLD, BEHRENDT, ULRICH
Format Patent
LanguageEnglish
French
Published 09.05.2006
Subjects
APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:The invention relates to a method for reduction of glucose consumption and/or lactate production during cultivation of animal cells, characterized in that cultivation is performed in the presence of a bi- or tricarbonic acid or a salt thereof at a concentration of about 1 to 580 mmol/l, whereby in the case where said di- or tricarbonic acid is citric acid or citrate this amount of said citric acid or citrate is not bound in chelate complex with iron or another transition metal ion.
Bibliography:Application Number: CA20032417689