COMPOSITIONS AND METHODS FOR GENERATING MONOCLONAL ANTIBODIES REPRESENTATIVE OF A SPECIFIC CELL TYPE

• Main Authors: BALD, LAURA N, STEPHAN, JEAN-PHILIPPE F, MATHER, JENNIE P, ROBERTS, PENELOPE R
• Format: Patent
• Language: English; French
• Published: 29.06.2000
• Edition: 7
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; HUMAN NECESSITIES; HYGIENE; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PHYSICS; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TESTING; VINEGAR; WINE

The present invention provides a method for generating a population of monoclonal antibodies capable of binding to antigens representative of a particular cell type. The method is unique in that it minimizes the generati on of non-representative antibodies cross-reacting with proteins not present in a particular cell type; it also maximizes the preservation of intact antigens, especially surface antigens, for production of a plurality of monoclonal antibodies that bind to the native antigens. The present invention also includes a method of determining the combination of cell surface antigens present on a specific cell type. Further provided by the invention are hybridomas, populations of monoclonal antibodies produced by the hybridomas of the present invention.