METHOD FOR PRODUCING TRANSGENIC PLANTS WITH MODIFIED 5-AMINOLEVULINIC ACID BIOSYNTHESIS, METHOD FOR IDENTIFYING 5-AMINOLEVULINIC ACID SYNTHESIS EFFECTORS

• Main Authors: DONN, GUNTER, SCHMIDT, FRANK
• Format: Patent
• Language: English; French
• Published: 17.06.1999
• Edition: 6
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PHYSICS; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TESTING; TRAPPING; VINEGAR; WINE

The invention relates to a method for producing transgenic plants by means of modified 5-aminolevulinic acid biosynthesis, transgenic plant cells, transgenic plant parts, transgenic seeds, and transgenic reproduction material. The invention is characterised in that one or several nucleic acid molecules coding for a protein with a 5-aminolevulinic acid synthase function (ALAS), selected from a group of feedback regulated ALAS, animal ALAS and bacterial ALAS, an active fragment thereof or an antisense or complementary sequence thereof, are integrated into the plant genome in a stable form. Effectors of natural 5-aminolevulinic acid biosynthesis in plants are found using a method for determining effector efficacy of a test substance in relation to a GSAAT, by: a) determining the enzymatic activity of the GSAAT in the absence of a test substance b) determining the enzymatic activity of the GSAAT in the presence of a test substance; and c) by comparing the enzymatic activities determined under a) and b).