METHODS FOR QUANTITATING HIGH-DENSITY LIPOPROTEIN CHOLESTEROL

Methods for quantitating HDL cholesterol, comprising adding to serum a surfactant selected from among polyoxyethylene alkylene phenyl ethers and polyoxyethylene alkylene tribenzylphenyl ethers and an enzyme reagent for assaying cholesterol, optionally together with a substance capable of inhibiting...

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Bibliographic Details
Main Authors HINO, KOICHI, NAKAMURA, MITSUHIRO, MANABE, MITSUHISA, NAKANISHI, KAZUO
Format Patent
LanguageEnglish
French
Published 04.03.1999
Edition7
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
ENZYMOLOGY
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
PHYSICS
PROCESSES OF PREPARING SUCH COMPOSITIONS
SPIRITS
TESTING
VINEGAR
WINE
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Summary:Methods for quantitating HDL cholesterol, comprising adding to serum a surfactant selected from among polyoxyethylene alkylene phenyl ethers and polyoxyethylene alkylene tribenzylphenyl ethers and an enzyme reagent for assaying cholesterol, optionally together with a substance capable of inhibiting the reaction of cholesterol in serum lipoproteins with the above enzyme reagent, and determining the amount of the cholesterol thus reacted within a period of time where cholesterol in HDL, among lipoproteins, preferentially reacts with the above enzyme reagent. By using this method, cholesterol in HDL can be conveniently and efficiently quantitated without resort to any pretreatment such as centrifugation. Thus, it is applicable to various automatic analyzers.
Bibliography:Application Number: CA19982301873