PLASMIDS FOR PLANT TRANSFORMATION AND METHOD FOR USING THE SAME

This invention relates to the Agrobacterium mediated plant transformation, especially to transformation of plants with T-DNA, where read-through at the borders is prohibited. This can be done by inhibition of transfer of vector DNA to the plant cell by creating DNA binding sites outside the T-border...

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Bibliographic Details
Main Authors ELZINGA, NICOLAS, SIMONS, LAMBERTUS HENRICUS, GODDIJN, OSCAR JOHANNES MARIA, DEKKER, BERNARDUS MARTINUS MARIA, PONSTEIN, ANNE SILENE, OHL, STEPHAN ANDREAS, STUIVER, MAARTEN HENDRIK, TIGELAAR, HENDRIK, HOEKSTRA, SIETSKE
Format Patent
LanguageEnglish
French
Published 14.01.1999
Edition7
Subjects
AGRICULTURE
ANIMAL HUSBANDRY
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FISHING
FORESTRY
HUMAN NECESSITIES
HUNTING
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
NEW PLANTS OR PROCESSES FOR OBTAINING THEM
PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
TRAPPING
VINEGAR
WINE
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Summary:This invention relates to the Agrobacterium mediated plant transformation, especially to transformation of plants with T-DNA, where read-through at the borders is prohibited. This can be done by inhibition of transfer of vector DNA to the plant cell by creating DNA binding sites outside the T-borders, but also to insert a coding sequence outside the T-borders which, whether or not in cooperation with genes from the host plant or genes cotransferred, are toxic to plants so that there is counterselection for plants with superfluous vector-DNA.
Bibliography:Application Number: CA19982294703