FATTY ACYL-COA: FATTY ALCOHOL ACYLTRANSFERASES

• Main Authors: LASSNER, MICHAEL W, LARDIZABAL, KATHRYN DENNIS, METZ, JAMES GEORGE
• Format: Patent
• Language: English; French
• Published: 29.11.2011
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRAPPING; VINEGAR; WINE

By this invention, nucleic acid sequences encoding for fatty acyl-CoA: fatty alcohol acyltransferase (wax synthase) are provided, wherein said wax synthase is active in the formation of a wax ester from fatty alcohol and fatty acyl-CoA substrates. Of special interest are nucleic acid sequences obtainable from a jojoba embryo wax synthase having an apparent molecular mass of approximately 33kD. Also considered are amino acid and nucleic acid sequences obtainable from wax synthase proteins and the use of such sequences to provide transgenic host cells capable of producing wax esters. La présente invention se rapporte à des séquences nucléotidiques codant l'acyle gras-CoA: alcool gras acyltransférase (paraffine-synthase), ladite paraffine-synthase étant active dans la formation d'un ester de paraffine à partir de substrats d'alcool gras et d'acyle gras-CoA. Les séquences nucléotidiques pouvant être obtenues à partir de la paraffine-synthase de cire de germe de jojoba ayant une masse moléculaire apparente d'environ 33 KD sont particulièrement intéressantes. Cette invention concerne également des séquences d'acides aminés et des séquences nucléotidiques pouvant être obtenues à partir de protéines de paraffine-synthase, et l'utilisation de telles séquences pour obtenir des cellules hôtes transgéniques capables de produire des esters de paraffine.