METHOD FOR PREPARING COMPOSITIONS FOR TRANSFERRING NUCLEIC ACIDS

• Main Authors: BOUKHNIKACHVILI, TSIALA, VACUS, JOEL
• Format: Patent
• Language: English; French
• Published: 16.04.1998
• Edition: 6
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

La présente invention concerne un procédé de préparation d'une composition pour le transfert d'acides nucléiques comprenant la mise en contact d'un acide nucléique avec un lipide cationique, selon lequel, préalablement à la mise en contact, le lipide cationique est soumis à une étape de chauffage. L'invention concerne également les compositions obtenues et leur utilisation. PCT No. PCT/FR97/01747 Sec. 371 Date Apr. 2, 1999 Sec. 102(e) Date Apr. 2, 1999 PCT Filed Oct. 3, 1997 PCT Pub. No. WO98/15639 PCT Pub. Date Apr. 16, 1998The invention concerns a method for preparing a composition for transferring nucleic acids consisting in contacting a nucleic acid with a cationic lipid, whereby, previous to contacting, the cationic lipid is subjected to a heating step. The invention also concerns the resulting compositions and their use.