METHODS AND COMPOSITIONS FOR IDENTIFYING RECEPTOR EFFECTORS

• Main Authors: TRUEHEART, JOSHUA, FUERNKRANZ, HANS A, HOLMES, SCOTT, PAUL, JEREMY I, NATHAN, DEBRA
• Format: Patent
• Language: English; French
• Published: 09.12.2003
• Edition: 6
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The present invention makes available a rapid, effective assay for screening and identifying pharmaceutically effective compounds that specifically interact with and modulate the activity of a cellular protein, e.g., a receptor or ion channel. The subject assay enables rapid screening of large numbers of compounds to identify those which act as an agonist or antagonist to the bioactivity of the cellular protein. The subject assay is particularl y amenable for identifying surrogate ligands for receptors especially from sma ll molecule or peptide libraries or from peptides produced by an autocrine system. Identification can be achieved via activation of a yeast signalling pathway, specially the yeast pheromone system pathway.