OPPOSABLE-ELEMENT ASSAY DEVICE EMPLOYING CONDUCTIVE BARRIER

A chromatographic assay device for use with immunoassays which allows rapid and convenient assays of analytes of biological interest, and permits extractions to be carried out in situ, avoiding the use of separate extraction vessels. The device has a wide dynamic range and avoids interference from p...

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Bibliographic Details
Main Author CHANDLER, HOWARD M
Format Patent
LanguageEnglish
French
Published 05.12.1996
Edition6
Subjects
CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PERFORMING OPERATIONS
PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
PHYSICS
TESTING
TRANSPORTING
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Summary:A chromatographic assay device for use with immunoassays which allows rapid and convenient assays of analytes of biological interest, and permits extractions to be carried out in situ, avoiding the use of separate extraction vessels. The device has a wide dynamic range and avoids interference from particulates or colored components. In one form, the device comprises: a first opposable component (12) comprising a sample preparation zone (18) adapted to receive a sample to be assayed; a second opposable component (14) comprising a chromatographic medium (20); and a conductive barrier (16) attached to the second opposable component. The first and second opposable components can be brought into opposition so as to cause the sample preparation zone to apply the sample to be tested to the chromatographic medium. Preferably, the analyte is detected with a visually detectable label. Other variations of the device vary the arangement of components to provide optimal chromatography for a variety of analytes. The devices can be incorporated in test kits, and assay methods, particularly sandwich immunoassays, using the devices are also disclosed.
Bibliography:Application Number: CA19962221125