ANTI-EGFR SINGLE-CHAIN FVS AND ANTI-EGFR ANTIBODIES

• Main Authors: PIULATS, JAIME, ADAN, JAIME, KETTLEBOROUGH, CATHERINE A, GUESSOW, DETLEF, MITJANS, FRANCESC, ROSELL, ELISABET, BENDIG, MARY M, BLASCO, FRANCESC, ANSELL, KEITH H
• Format: Patent
• Language: English; French
• Published: 21.09.1995
• Edition: 6
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; HUMAN NECESSITIES; HYGIENE; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PHYSICS; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; TESTING; VINEGAR; WINE

This invention relates to new anti-EGFR antibodies and single-chain Fvs (scFvs) thereof which can be obtained from phage-antibody libraries constructed from cells of an immunized mammalian, preferably a mouse. Two of the single-chain Fvs isolated from the phageantibody libraries were engineered to create partially humanized whole antibody molecules These chimeric anti-EGFR antibodies contain constant regions of human immunoglobulins and can be used as well as the single-chain Fvs as agents for the diagnosis and therapy of human tumors.