EXPRESSION SYSTEM FOR PRODUCING APOLIPOPROTEIN AI-M

The invention relates to an expression system giving high extracellar production of apolipoprotein AI-M (Milano) using E. coli and comprises a plasmid carrying an origin of replication,an inducible promoter sequence, a DNA sequence coding for a signal peptide, a DNA sequence coding for apolipoprotei...

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Bibliographic Details
Main Authors SEJLITZ, TORSTEN, KALDEREN, CHRISTINA, LAKE, MATS, MIKAELSSON, SA, ABRAHMSEN, LARS, HOLMGREN, ERIK
Format Patent
LanguageEnglish
French
Published 23.06.1994
Edition6
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
ORGANIC CHEMISTRY
PEPTIDES
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:The invention relates to an expression system giving high extracellar production of apolipoprotein AI-M (Milano) using E. coli and comprises a plasmid carrying an origin of replication,an inducible promoter sequence, a DNA sequence coding for a signal peptide, a DNA sequence coding for apolipoprotein AI-M, and a transcription terminatior. The invention also relates to a metod of producing apolipoprotein AI-M using the expression system.
Bibliography:Application Number: CA19932150928